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Immobilization of Cr(VI) and Its Reduction to Cr(III) Phosphate by Granular Biofilms Comprising a Mixture of Microbes▿

机译:含微生物混合物的颗粒生物膜固定Cr(VI)及其还原为磷酸Cr(III))

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摘要

We assessed the potential of mixed microbial consortia, in the form of granular biofilms, to reduce chromate and remove it from synthetic minimal medium. In batch experiments, acetate-fed granular biofilms incubated aerobically reduced 0.2 mM Cr(VI) from a minimal medium at 0.15 mM day−1 g−1, with reduction of 0.17 mM day−1 g−1 under anaerobic conditions. There was negligible removal of Cr(VI) (i) without granular biofilms, (ii) with lyophilized granular biofilms, and (iii) with granules in the absence of an electron donor. Analyses by X-ray absorption near edge spectroscopy (XANES) of the granular biofilms revealed the conversion of soluble Cr(VI) to Cr(III). Extended X-ray absorption fine-structure (EXAFS) analysis of the Cr-laden granular biofilms demonstrated similarity to Cr(III) phosphate, indicating that Cr(III) was immobilized with phosphate on the biomass subsequent to microbial reduction. The sustained reduction of Cr(VI) by granular biofilms was confirmed in fed-batch experiments. Our study demonstrates the promise of granular-biofilm-based systems in treating Cr(VI)-containing effluents and wastewater.
机译:我们评估了颗粒状生物膜形式的混合微生物聚生体减少铬酸盐并将其从合成基本培养基中去除的潜力。在分批实验中,以醋酸盐喂养的颗粒状生物膜在0.15 mM day-1 g-1下从基本培养基中需氧培养降低了0.2 mM Cr(VI),在厌氧条件下减少了0.17 mM day-1 g-1。 Cr(VI)的去除几乎可以忽略不计(i)没有颗粒状生物膜,(ii)冻干的颗粒状生物膜和(iii)在没有电子供体的情况下具有颗粒。通过X射线吸收近边缘光谱(XANES)对颗粒状生物膜的分析表明,可溶性Cr(VI)转化为Cr(III)。含铬颗粒生物膜的扩展X射线吸收精细结构(EXAFS)分析表明与磷酸Cr(III)相似,表明微生物还原后,Cr(III)被磷酸盐固定在生物质上。补料分批实验证实了颗粒状生物膜对Cr(VI)的持续还原作用。我们的研究表明,基于颗粒生物膜的系统有望用于处理含六价铬的废水和废水。

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